Molecular Detection and Differentiation of Hypoderma Species (Diptera: Oestridae) Affecting Cattle in Pakistan
نویسندگان
چکیده
Hypoderma spp. larvae are the cause of internal myiasis in different animals which is characterized by subcutaneous warbles. These are mostly differentiated at species level based on the morphology of third stage larvae. Therefore, the present study was conducted to amplify the most variable part of the cox1 gene of two Hypoderma spp. (i.e., Hypoderma bovis and H. lineatum) retrieved in Pakistan by PCR. This part encodes the region spanning from external loop 4 (E4) to carboxylic terminal. The nucleotide and amino acidic identity within the cox1 gene of the two Hypoderma spp. were 89.5% and 93.4%, respectively. Particularly, the amino acid sequences of the two Hypoderma spp. herein analyzed were identical to that available in GenBankTM (H. bovis, GU984818 and H. lineatum, GU584123). The mean nucleotide composition was A=32.7%, C=16.6%, G=11.5% and T= 39.2%. The overall transition/transversion (Ts/Tv) ratio (R) was 1.008. In addition, the molecular analysis of PCR products was also performed. Nucleotide sequence changes and inter-specific variations will help in genetic differentiation of the examined species providing information for rapid molecular identification of Hypoderma spp.
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